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J Exerc Rehabil > Volume 10(1);2014 > Article
Yun, Park, Ji, Kim, Ko, Kim, and Kim: Treadmill exercise ameliorates symptoms of attention deficit/hyperactivity disorder through reducing Purkinje cell loss and astrocytic reaction in spontaneous hypertensive rats

Abstract

Attention deficit/hyperactivity disorder (ADHD) is a neurobehavioral disorder of cognition. We investigated the effects of treadmill exercise on Purkinje cell and astrocytic reaction in the cerebellum of the ADHD rat. Adult male spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKYR) weighing 210± 10 g were used. The animals were randomly divided into four groups (n= 15): control group, ADHD group, ADHD and methylphenidate (MPH)-treated group, ADHD and treadmill exercise group. The rats in the MPH-treated group as a positive control received 1 mg/kg MPH orally once a day for 28 consecutive days. The rats in the treadmill exercise group were made to run on a treadmill for 30 min once a day for 28 days. Motor coordination and balance were determined by vertical pole test. Immunohistochemistry for the expression of calbindinD-28 and glial fibrillary acidic protein (GFAP) in the cerebellar vermis and Western blot for GFAP, Bax, and Bcl-2 were conducted. In the present results, ADHD significantly decreased balance and the number of calbindin-positive cells, while GFAP expression and Bax/Bcl-2 ratio in the cerebellum were significantly increased in the ADHD group compared to the control group (P< 0.05, respectively). In contrast, treadmill exercise and MPH alleviated the ADHD-induced the decrease of balance and the number of calbindine-positive cells, and the increase of GFAP expression and Bax/Bcl-2 ratio in the cerebellum (P< 0.05, respectively). Therefore, the present results suggested that treadmill exercise might exert ameliorating effect on ADHD through reduction of Purkinje cell loss and astrocytic reaction in the cerebellum.

INTRODUCTION

Attention deficit/hyperactivity disorder (ADHD) is one of the most common childhood neurobehavioral disorders characterized by hyperactivity, impulsivity, and attention deficit (Banaschewski et al., 2004). It affects approximately 5–12% of children (Bedford et al., 1979; Berridge et al., 2006) which frequently persist into adolescence and adulthood (Banaschewski et al., 2004; Bethancourt et al., 2009). For this reason, the etiology of ADHD has been extensively studied using animal and humans (Kim et al., 2011; Passarelli et al., 2013; Wang et al., 2013) and then the pathophysiology of ADHD are suggested to be closely associated with anatomical and functional abnormalities in frontal-striatal-cerebellar circuits (Giedd et al., 2001). Of these circuits, many studies have shown that cerebellum is one of the most important parts of the brain related to the ADHD.
The cerebellum is as a key anatomical structure underlying normal attentional and cognitive control mechanisms. Previous studies have shown that the cerebellum is thought to play a crucial role in cognitive function, particularly in learning, attention, and emotional regulation (Ivry et al., 2002; Schmahmann and Sherman 1998). On the other hand, cerebellar impairment may contribute to the cerebellum cognitive and affective syndrome (CCAS) (De Smet et al., 2013). Related to the ADHD, several studies have reported that the symptoms of ADHD has strongly related to the dysfunction of cerebellar circuits (Giedd et al., 2001) induced by reduction of cerebellar volume (Berquin et al., 1998; Castellanos et al., 2002; Semrud-Clikeman et al., 2000). In addition, the reduced size of cerebellar vermis was associated with the clinical deficits by ADHD (Ashtari et al., 2005; Buderath et al., 2009; Mackie et al., 2007). Recently, both functional and morphometric cerebellar abnormalities have been consistently demonstrated in ADHD (Soliva et al., 2009).
Purkinje neurons in the cerebellum play crucial role in movement, coordination, and cognitive function including motor learning (Passarelli et al., 2013; Seo et al., 2010; Zhang et al., 2013). It has been reported that the impairment of Purkinje cells causes cerebellar ataxia (Jones et al., 2010; Lu et al., 2011). In addition, loss of Purkinje cells is related to the brain disorder such as Alzeheimer’s disease and ADHD (Fukutani et al., 1996; Passarelli et al., 2013). Despite such findings involving the cerebellum in response to a brain disorder, the influence of ADHD on Purkinje cell loss and astrogliosis in the cerebellum remains unclear.
In clinic, pharmacological stimulants such as norepinephrine agonists (Banaschewski et al., 2004) and methylphenidate (MPH) and indirect dopaminergic agonist were used to reduce the symptoms of ADHD. Of them, MPH is the most popular medication because of its effects on ADHD (Bethancourt et al., 2009). These stimulants, however, sometimes exert no effectiveness or induce side effect, often restrain their continued use (Banaschewski et al., 2004). For this reason, the physical activity or regular exercise get attention as a kind of non-medicinal effective method for reducing the symptom of ADHD.
Many studies have reported that exercise improves motor performance, learning, memory, and cognitive function in animals (Erickson et al., 2013; Jee et al., 2008; Kim et al., 2010a; Radak et al., 2001). Related to the Purkinje cells of cerebellum, treadmill exercise increased dendritic spine plasticity by inducing the changes of Purkinje cell in adult rat (González-Burgos et al., 2011). Another study also showed that physical exercise also improved working speed and social behavioral problems, and diminished hyperactivity in ADHD children (Majorek et al., 2004).
Although the beneficial effects of physical exercise on abnormal brain function are well documented, there is no available study for the effect of exercise on ADHD in relation with Purkinje cells and astrocytic reaction in the cerebellum. Therefore, we investigated the effects of treadmill exercise on Purkinje cell loss and astrocyte activation in the cerebellum of the ADHD rat.

MATERIALS AND METHODS

Experimental animals and treatments

Adult male spontaneously hypertensive rats (SHR) weighing 210±10 g were used as the ADHD animal model, because the SHR displays the major symptoms of ADHD such as hyperactivity. SHR was selected only when showed hyperactivity in open field test. Meanwhile, Wistar-Kyoto rats (WKYR) weighing 210±10 g were used as the control in this study, according to the previous study (Sagvolden, 2000). The rats were housed under controlled temperature (20±2°C) and lighting conditions (07:00 to 19:00), with food and water made available ad libitum throughout the experiments. Prior to the exercise and drug treatment, we tested the effects of different amount of treadmill exercise on the hyperactivity, as pre-test. The animals were randomly divided into four groups (n=15): control group, ADHD group, ADHD and methylphenidate (MPH)-treated group, ADHD and treadmill exercise group. The rats in the MPH-treated group received 1 mg/kg MPH (Ritalin®, Novartis Co., Bassel, Switzerland) orally once a day for 28 consecutive days, according to the previous study (Lijffijtet et al., 2005). All animal procedures were performed in accordance with the animal care guidelines of the National Institutes of Health (NIH) and the Korean Academy of Medical Sciences.

Vertical pole test

For the vertical pole test, the animal was placed face up on a cloth-tape-covered pole (3.0 cm diameter, 150 cm length), which was held in a horizontal position, then the pole was gradually lifted to a vertical position and the time a rat stayed on the pole was recorded for a maximum of 180 sec. In this test, the animal with deficits in motor coordination and balance will fall off the pole (Tanriover et al., 2010).

Treadmill exercise protocol

The rats in the treadmill exercise group were subjected to run on a treadmill for 30 min once a day, five times a week, for 28 days. Exercise load for the running group consisted of running at a speed of 2 m/min for the first 5 min, at a speed of 5 m/min for the next 5 min, and then at a speed of 8 m/min for the last 20 min, with the 0° inclination. This intensity corresponded to the low-intensity treadmill exercise (% maximal oxygen consumption) of rats in this age (Bedford et al., 1979).

Tissue preparation

The rats were sacrificed 29 days after the starting of experiment, immediately after vertical pole test. To begin the sacrifice process, animals were fully anesthetized using Zoletil 50® (10 mg/kg, i.p.; Vibac Laboratories, Carros, France). The anesthetized rats were transcardially perfused with 50 mM phosphate-buffered saline (PBS), and fixed with a freshly prepared solution consisting of 4% paraformaldehyde (PFA) in 100 mM phosphate buffer (PB) at pH 7.4. Cerebellums were dissected, post fixed in the same fixative overnight, and transferred to 30% sucrose solution for cryoprotection. Sagittal sections of 40 μm thickness in each section of cerebellum were made with a freezing microtome (Leica, Nussloch, Germany).

Immunohistochemistry for calbindinD-28k and GFAP

For the detection of calbindin-positive and glial fibrillary acidic protein (GFAP)-positive cells in the cerebellar vermis, immunohistochemistry was performed. Briefly, the sections were incubated in PBS for 10 min and washed 3 times with PBS, and then incubated in 1% hydrogen peroxide (H2O2) for 30 min. Next, the sections were incubated overnight with mouse anti calbindin D-28k antibody (Sigma Chemical Co., St. Louis, MO, USA) at a dilution of 1:1,000 for visualization of Purkinje cells or with mouse anti-GFAP antibody (Chemicon, Temecula, CA, USA) at a dilution of 1:2,000 for visualization of astrocytes. The sections were then incubated for 1 h with anti-mouse secondary antibody (1:200; Vector Laboratories, Burlingame, CA, USA). Bound secondary antibody was then amplified with a Vector Elite ABC kit® (Vector Laboratories). The sections were subsequently incubated with avidin-biotin-peroxidase complex (1:100; Vector Laboratories) for 1 h at room temperature. Immunoreactivity was visualized by incubating the sections in a solution consisting of 0.05% 3,3-diaminobenzidine (DAB) and 0.01% H2O2 in 50 mM Tris buffer (pH 7.6) for approximately 3 min. The sections were then mounted on gelatin-coated glass slides. The slides were air-dried overnight at room temperature, and the coverslips were mounted using Permount®.

Western blot analysis

Brains were collected at lobules VIII–X areas of the cerebellum, and then were immediately frozen at −70°C. Cerebellums were homogenized on ice, and lysed in a lysis buffer containing 50 mM HEPES (pH 7.5), 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1 mM EGTA, 1.5 mM MgCl2 ·6H2O, 1 mM sodium orthovanadate, and 100 mM sodium flouride. Protein content was measured using a Bio-Rad colorimetric protein assay kit (Hercules, CA, USA). Protein (30 μg) was separated on SDS-polyacrylamide gels and transferred onto a nitrocellulose membrane. Mouse-actin antibody (1:3,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse GFAP antibody (1:1,000; Santa Cruz Biotechnology), mouse Bax antibody (1:1,000; Santa Cruz Biotechnology), and mouse Bcl-2 antibody (1:1,000; Santa Cruz Biotechnology) were used as the primary antibodies. Horseradish peroxidase-conjugated anti-mouse antibody for actin, AP, Bax and Bcl-2 (1: 3,000; Vector Laboratories) were used as the secondary antibodies. Experiments were performed in normal laboratory conditions and at room temperature, except for the transferred membranes. Transferred membranes were performed at 4°C with the cold pack and pre-chilled buffer. Band detection was performed using the enhanced chemiluminescence (ECL) detection kit (Santa Cruz Biotechnology). To compare the relative expression of proteins, the detected bands were calculated densitometrically using Image-Pro® Plus software (Media Cybernetics, Bethesda, MD, USA).

Data analysis

To quantify the number of Purkinje neurons three and four non-adjacent vermis sections per animal were chosen. Using Image-Pro® Plus software (Media Cybernetics, Bethesda, MD), a 1,000 μm contour line was drawn along the Purkinje neuron layer on both sides of the fissure or gyrus. The number of Purkinje neurons for each sample was calculated as the average number of Purkinje neurons. Before beginning the image analysis, the light source was adjusted to the brightness generating the best possible contrast between positive and negative staining astrocytes. Quantification for Purkinje neurons was conducted in a blinded manner. All data are presented as the mean standard error of the mean (SEM). Statistical analysis for Purkinje cell quantification was done by ANOVA following Duncan’s post hoc test using SPSS computer software (Version 18.0). The differences were considered significant at P <0.05.

RESULTS

Effect of treadmill exercise on balance in the vertical pole test

The vertical pole test was performed for 4 weeks after starting of experiment. The time score of the vertical pole test are presented in Fig. 1. The time score was 154.60±6.19 sec in the control group, 57.60±3.20 sec in the ADHD group, 149.80±20.08 sec in the ADHD and MPH-treated group, and 137.60±8.85 sec in the ADHD and treadmill exercise group. These results showed that the balance of the ADHD rats was lower than that of the control rats (P <0.05). In contrast, treadmill exercise and MPH treatment significantly increased the balance of ADHD rats (P <0.05).

Effect of treadmill exercise on survival of Purkinje cells in the cerebellum

The photomicrographs of the Purkinje neuronal cells in the gyrus of lobules VIII of the cerebellar vermis (dotted rectangle) are presented in Fig. 2A. The number of calbindin-positive Purkinje cells in the gyrus of lobules VIII of the cerebellar vermis was 56.85 ±1.51/section in the control group, 27.23±1.29/section in the ADHD group, 47.01±2.27/section in the ADHD and MPH-treated group, and 44.28±0.83/section in the ADHD and treadmill exercise group (Fig 2B). These results showed that the number of calbindin-positive cells in the cerebellum of the ADHD rats was significantly decreased compared to that of the control rats (P <0.05), and that treadmill exercise and MPH treatment significantly prevented death of Purkinje neurons (P <0.05).

Effect of treadmill exercise on GFAP protein expression in the cerebellum

The photomicrographs of GFAP-positive cells in both the granule layers of cerebellar vermis are presented in Fig. 3A. The level of GFAP protein in the cerebellum was analyzed by Western blotting (Fig. 3B). When the level of mature GFAP (55 kDa) in the control group was set as 1.00±0.0, the level of mature GFAP was 1.43±0.33 in the ADHD group, 1.00±0.00 in the ADHD and MPH-treated group, and 1.02±0.06 in the ADHD and treadmill exercise group. The number of GFAP-positive cells in the gyrus of lobules VIII of the cerebellar vermis was increased when compared to the control group. In contrast, the GFAP-positive cells was decreased by treadmill exercise and MPH treatment (Fig. 3C). These results showed that the GFAP expression in the cerebellums of the ADHD rats was significantly increased compared to that of the control rats (P <0.05), and that treadmill exercise and MPH treatment significantly decreased GFAP expression in the cerebellum (P <0.05).

Effect of treadmill exercise on Bax and Bcl-2 protein expression in the cerebellums

The level of Bax and Bcl-2 protein in the cerebellum were analyzed by Western blotting (Fig. 4A). Bax expression is presented in Fig 4B. When the level of mature Bax (21 kDa) in the control group was set as 1.00±0.0, the level of mature Bax was 2.83±0.18 in the ADHD group, 1.22±0.18 in the ADHD and MPH-treated group, and 1.45±0.39 in the ADHD and treadmill exercise group. Bcl-2 expression is presented in Fig 4C. When the level of mature Bcl-2 (26 kDa) in the control group was set as 1.00 ±0.0, the level of mature Bcl-2 was 0.43±0.10 in the ADHD group, 0.92±0.09 in the ADHD and MPH-treated group, and 0.86±0.13 in the ADHD and treadmill exercise group. The ratio of Bax/Bcl-2 protein expression is presented in Fig. 4D. When the level of mature Bax/Bcl-2 ratio in the control group was set as 1.00±0.0, the level of mature Bax/Bcl-2 ratio was 10.84±2.79 in the ADHD group, 1.50±0.27 in the ADHD and MPH-treated group, and 2.38±0.73 in the ADHD and treadmill exercise group. These results showed that the expression of Bax protein in the ADHD rats was significantly higher than that in the control rats (P < 0.05), while treadmill exercise and MPH treatment suppressed the Bax protein expression in the cerebellum (P <0.05). The expression of Bcl-2 protein in the ADHD rats was significantly lower than that in the control rats (P <0.05), while treadmill exercise and MPH treatment enhanced the Bcl-2 protein expression in the cerebellum (P <0.05). The ratio of Bax/Bcl-2 protein expression in the ADHD rats was significantly higher than that in the control rats (P <0.05), while treadmill exercise and MPH treatment suppressed the ratio of Bax/Bcl-2 protein expression in the cerebellum (P <0.05).

DISCUSSION

The cerebellum is a major structures involved in motor learning, motor coordination and control by maintaining a balance and muscle tension (Ito, 2000; Lee et al., 2007; Thompson et al., 1995). A few studies suggested that dysregulation within cerebellar circuits may contribute to the symptoms of ADHD such as attention deficit and impairment of motor coordination (Buderath et al., 2009; Hart et al., 2012). Buderath et al. (2009) reported that cerebellar dysfunction in children with ADHD induces the postural and gait deficits such as static and dynamic balance. Hart et al. (2012) also showed that consistent and replicable deficits of timing function were observed in patients with ADHD. In this study, ADHD rats showed significantly lower balance than the control rats in the vertical pole test. Together with previous studies, our result supported the hypothesis that ADHD may be closely associated with the dysfunction of cerebellum leading to motor deficit like balance problem.
Although the precise mechanism on occurrence of cerebellar dysfunction by ADHD was not fully understood to date, previous studies reported the relevance that the Purkinje cells of cerebellar cortex is closely related to the motor function (Black et al., 1990; Kleim et al., 1998; Zhang et al., 2013). Black et al. (1990) showed that the increased number of synapses of Purkinje cell was attributable to the increase of motor learning. Kleim et al. (1998) reported that the learned complex motor skills increase the number of synapses on Purkinje cells in the adult rats. In contrast, Zhang et al. (2013) supported the idea that impairment of motor coordination and motor learning is closely associated with the dysfunction of cerebellar Purkinje cells in mouse. In particular, several structural imaging studies have consistently reported that the cerebellar abnormalities such as reduced volume and smaller anatomic areas of cerebellum were observed in adult and children with ADHD (Ashtari et al., 2005; Castellanos et al., 2002; Semrud-Clikeman et al., 2000). Recently, Passarelli et al. (2013) suggested that the immune dysregulation of the Purkinje cell of the cerebellum is associated with the onset of ADHD in children. Similar to the previous studies, we observed the increased expressions of apoptotic factors such as Bax, Bcl-2 and the increased astrocytic factor such as GFAP in Purkinje cells of the cerebellar vermis in ADHD group. That is, ADHD increased apoptosis and astrogliosis in Purkinje cell of the cerebellum, which is induced Purkinje cell loss in the cerebellum. Therefore, our results supported the idea that the symptoms of ADHD might be induced by the death or dysfunction of Purkinje neurons in part.
It has been widely accepted that regular exercise plays a crucial role to enhance brain structure and cognitive function. Regular exercise increased neuronal survival, learning, and memory (Chae et al., 2012; Kim et al., 2010a, 2010b). It also alleviated traumatic brain injury (Seo et al., 2010), and reduced the symptoms of brain disorder such as Parkinson’s disease, Alzheimer’s disease, and ADHD (Kim et al., 2011; Majorek et al., 2004; O’Dell et al., 2007; Petzinger et al., 2007). In addition, several studies demonstrate that regular exercise enhanced attention and concentration performance (Budde et al., 2008; Hillman et al., 2009). Related to ADHD, a few studies have reported the possible effect of exercise on ADHD (Gapin and Etnier, 2010; Majorek et al., 2004; Medina et al., 2010; Park et al., 2013; Tantillo et al., 2002). Tantillo et al. (2002) suggested that motor impersistence was decreased by maximal exercise in boys with ADHD. Majorek et al. (2004) showed that physical movement improved working speed and diminished hyperactivity in children with ADHD. Similar to the previous studies, we demonstrated that treadmill exercise recovered balance ability through the reduction of Purkinje cell loss and astrocytic reaction in the cerebellar vermis in rat with symptoms of ADHD and its effect indicated similar level with medication, MPH. These results supported the possibility that regular exercise might be an effective interventional program to reduce problematic behaviors in ADHD through inhibiting Purkinje cell loss in cerebellum.
Although the interesting results of treadmill exercise on ADHD, there was limitation of the current study. Primary limitation is that there is no criteria on the level of balance ability for ADHD animal model. For this reason, the present behavioral results did not explain directly whether the decreased level of balance ability in ADHD group is associated with symptoms of ADHD or not. It is required to examine various types of behaviors for searching adequate behavior test. In sum, treadmill exercise might exert ameliorating effect on ADHD through reduction of Purkinje cell loss and astrocytic reaction in the cerebellum.

ACKNOWLEDGMENTS

This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2011-327-G00121).

Notes

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

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Fig. 1.
Effects of treadmill exercise on vertical pole test. (CON) control group, (ADHD) attention deficit/hyperactivity disorder group, (ADHD+MPH), attention deficit/hyperactivity disorder and methylphenidate-treated group, (ADHD+EXE), attention deficit/hyperactivity disorder and treadmill exercise group. *P< 0.05 compared with the control group. #P< 0.05 compared with the ADHD group. The data are expressed as the mean± SEM.
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Fig. 2.
Effects of treadmill exercise on surviving Purkinje cells in the cerebellular vermis. (A) Photomicrographs of a sagittal section Purkinje cells in the cerebellum. The sections were stained for Purkinje cells immunoreactivity with calbindin D-28k (brown). The scale bar represents 200 μm. (B) The mean number of calbindin-positive Purkinje cells in each group. (CON) control group, (ADHD) attention deficit/hyperactivity disorder group, (ADHD+MPH), attention deficit/hyperactivity disorder and MPH-treated group, (ADHD+EXE), attention deficit/hyperactivity disorder and treadmill exercise group. *P< 0.05 compared with the control group. #P< 0.05 compared with the ADHD group. The data are expressed as the mean± SEM.
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Fig. 3.
Effects of treadmill exercise on activation of the reactive astrocytes in the cerebellular vermis. (A) Photomicrographs of GFAP-positive reactive astrocytes in molecular layer of the cerebellum. The sections were stained for GFAP immunoreactivity (brown). The scale bar represents 200 μm. (B) Western blot analysis of GFAP protein in the cerebellar vermis. (C) The mean optical density of reactive astrocytes in each group. (CON) control group, (ADHD) attention deficit/hyperactivity disorder group, (ADHD+MPH), attention deficit/hyperactivity disorder and MPH-treated group, (ADHD+EXE), attention deficit/hyperactivity disorder and treadmill exercise group. *P< 0.05 compared with the control group. #P< 0.05 compared with the ADHD group. The data are expressed as the mean± SEM.
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Fig. 4.
Effects of treadmill exercise on the expression of Bcl-2 and Bax protein. (A) Western blot analysis of Bcl-2 and Bax protein in the cerebellum. (B) The mean optical density of Bax expression in each group. (C) The mean optical density of Bcl-2 expression in each group. (D) The Bax/Bcl-2 ratio in each group. (CON) control group, (ADHD) attention deficit/ hyperactivity disorder group, (ADHD+MPH), attention deficit/hyperactivity disorder and MPH- treated group, (ADHD+EXE), attention deficit/hyperactivity disorder and treadmill exercise group. *P< 0.05 compared with the control group. #P< 0.05 compared with the ADHD group. The data are expressed as the mean± SEM.
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